Techniques

We have expertise in various molecular biology techniques (eg, cloning, PCR, real time quantitative PCR, site‐directed mutagenesis, next generation sequencing), cell biology (cell culture, immunofluorescent staining, live cell imaging, RNAi, transfection, lentivirus production, isolation of primary neuronal cells, prion infection assays), protein chemistry (recombinant protein expression and purification, protein gels, western immunoblotting, biophysical techniques), and general biochemical assays. We have expertise in the isolation and deep sequencing of RNA isolated from exosomes using both the SOLiD and Ion Torrent sequencing platforms. We also use cryo-electron microscopy to image exosomes and microvesicles.